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rabbit polyclonal antibody against pcna  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal antibody against pcna
    Rabbit Polyclonal Antibody Against Pcna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+pcna/PCNA+Antibody/pmc06112232-74-54-62
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibody against pcna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Hippocampal Neurogenesis Levels Predict WATERMAZE Search Strategies in the Aging Brain
    Article Snippet: .. After quenching with 3% H 2 O 2 /10% methanol in 0.1 M TBS for 15 minutes and pre-incubating with 5% normal goat serum (NGS) for 1 hour, the sections were incubated for 48 hours at 4°C with a rabbit polyclonal anti-Ki-67 primary antibody (1:500; Vector Laboratories, Burlingame, CA, USA) or a rabbit polyclonal antibody against PCNA (1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. After thorough rinsing the sections were then incubated for 2 hours with a biotin-conjugated goat anti-rabbit IgG secondary antibody (1:200; Vector Laboratories).

    Article Title: Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington's Disease
    Article Snippet: .. After quenching with 3% H 2 O 2 /10% methanol in 0.1 m PBS for 15 min and preincubation with 5% normal goat serum (NGS) for 1 h, sections were incubated for 48 h at 4°C with either a rabbit polyclonal anti-Ki-67 primary antibody (1 : 500; Vector Laboratories, Burlingame, CA, USA) or a rabbit polyclonal antibody against PCNA (1 : 100; Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Article Title: The antidepressant-like effect of chronic guanosine treatment is associated with increased hippocampal neuronal differentiation.
    Article Snippet: Guanosine is a purine nucleoside that occurs naturally in the central nervous system, exerting trophic effects.. Given its neuroprotective properties, the potential of guanosine as an antidepressant has been recently examined.. Within this context, the present study sought to investigate the effects of chronic treatment with guanosine on the tail suspension test (TST), open field test and adult hippocampal neurogenesis.

    Incubation:

    Article Title: Hippocampal Neurogenesis Levels Predict WATERMAZE Search Strategies in the Aging Brain
    Article Snippet: .. After quenching with 3% H 2 O 2 /10% methanol in 0.1 M TBS for 15 minutes and pre-incubating with 5% normal goat serum (NGS) for 1 hour, the sections were incubated for 48 hours at 4°C with a rabbit polyclonal anti-Ki-67 primary antibody (1:500; Vector Laboratories, Burlingame, CA, USA) or a rabbit polyclonal antibody against PCNA (1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. After thorough rinsing the sections were then incubated for 2 hours with a biotin-conjugated goat anti-rabbit IgG secondary antibody (1:200; Vector Laboratories).

    Article Title: Human colorectal cancer cells induce vascular smooth muscle cell apoptosis in an exocrine manner
    Article Snippet: The lysates containing equal amounts of protein were separated by SDS-PAGE, and electroblotted onto polyvinylidene difluoride membranes by a semi-dry transfer system (Bio-Rad). .. Blots were blocked with 5% nonfat dry milk in TBST (10 mM Tris–Cl, pH 7.5, 150 mM NaCl, 0.1% Tween-20) for 2 h at room temperature, then probed with mouse monoclonal antibody against β-actin (1:800, Santa Cruz), mouse polyclonal antibody against Bcl-2(1:800, ABclonal), rabbit polyclonal antibody against Bax (1:1000, ABclonal), rabbit polyclonal antibody against PCNA (1:1000, Santa Cruz) and rabbit polyclonal antibody against Caspase-3 (1:800, Cell Signaling Technology) at 4°C overnight, then washed with TBST and incubated with fluorescence-conjugated goat antimouse/rabbit IgG (Rockland) for 1 h at room temperature. .. The immunoreactive bands were visualized on Odyssey Infrared Imaging System (Li-Cor Biosciences), and the protein expression was quantified by densitometric analysis using Odyssey Imaging Software v3.0 (Li-Cor Biosciences).

    Article Title: Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington's Disease
    Article Snippet: .. After quenching with 3% H 2 O 2 /10% methanol in 0.1 m PBS for 15 min and preincubation with 5% normal goat serum (NGS) for 1 h, sections were incubated for 48 h at 4°C with either a rabbit polyclonal anti-Ki-67 primary antibody (1 : 500; Vector Laboratories, Burlingame, CA, USA) or a rabbit polyclonal antibody against PCNA (1 : 100; Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Article Title: The antidepressant-like effect of chronic guanosine treatment is associated with increased hippocampal neuronal differentiation.
    Article Snippet: Guanosine is a purine nucleoside that occurs naturally in the central nervous system, exerting trophic effects.. Given its neuroprotective properties, the potential of guanosine as an antidepressant has been recently examined.. Within this context, the present study sought to investigate the effects of chronic treatment with guanosine on the tail suspension test (TST), open field test and adult hippocampal neurogenesis.

    Fluorescence:

    Article Title: Human colorectal cancer cells induce vascular smooth muscle cell apoptosis in an exocrine manner
    Article Snippet: The lysates containing equal amounts of protein were separated by SDS-PAGE, and electroblotted onto polyvinylidene difluoride membranes by a semi-dry transfer system (Bio-Rad). .. Blots were blocked with 5% nonfat dry milk in TBST (10 mM Tris–Cl, pH 7.5, 150 mM NaCl, 0.1% Tween-20) for 2 h at room temperature, then probed with mouse monoclonal antibody against β-actin (1:800, Santa Cruz), mouse polyclonal antibody against Bcl-2(1:800, ABclonal), rabbit polyclonal antibody against Bax (1:1000, ABclonal), rabbit polyclonal antibody against PCNA (1:1000, Santa Cruz) and rabbit polyclonal antibody against Caspase-3 (1:800, Cell Signaling Technology) at 4°C overnight, then washed with TBST and incubated with fluorescence-conjugated goat antimouse/rabbit IgG (Rockland) for 1 h at room temperature. .. The immunoreactive bands were visualized on Odyssey Infrared Imaging System (Li-Cor Biosciences), and the protein expression was quantified by densitometric analysis using Odyssey Imaging Software v3.0 (Li-Cor Biosciences).

    Western Blot:

    Article Title: Enforced GFI1 expression impedes human and murine leukemic cell growth
    Article Snippet: Primary human CD34 + cells, AML cell lines transduced with eGFP and GFI1-eGFP lentiviral vectors were sorted and nuclear proteins were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, Darmstadt, Germany). .. Antibodies used for Western blotting included goat polyclonal antibody against GFI1 (N-20) (1:1000, G6670, Sigma-Aldrich, Darmstadt, Germany), rabbit polyclonal antibody against PCNA (1:500, sc-7907, Santa Cruz Biotechnologies, Minneapolis, USA), donkey anti-goat IgG-HRP (1:5000, sc-2020, Santa Cruz Biotechnologies) and Goat anti-rabbit IgG-HRP (1:2500, sc-2030, Santa Cruz Biotechnologies). .. ECL Western blot analysis system (Amersham) was used for detection.



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    Image Search Results


    Inhibition of melanoma cells proliferation in vivo by HPL, 3HFWC, and HPL+3HFWC treatments, as demonstrated by ( A ) PCNA immunoexpression (green) in melanoma tissue, red signal—propidium iodide nuclear staining (scale bar and orig. magnification—25 μm, × 63); ( B ) PCNA nuclear immunopositivity levels (0/+ no/weak immunopositivity; ++/+++ medium/strong immunopositivity of nuclei); and ( C ) mitotic index in melanoma tissue. Mean values ± SEM; statistical significance in comparison to melanoma tissue of control animals (* p value) and between the treated groups.

    Journal: Nanomaterials

    Article Title: Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo

    doi: 10.3390/nano13030372

    Figure Lengend Snippet: Inhibition of melanoma cells proliferation in vivo by HPL, 3HFWC, and HPL+3HFWC treatments, as demonstrated by ( A ) PCNA immunoexpression (green) in melanoma tissue, red signal—propidium iodide nuclear staining (scale bar and orig. magnification—25 μm, × 63); ( B ) PCNA nuclear immunopositivity levels (0/+ no/weak immunopositivity; ++/+++ medium/strong immunopositivity of nuclei); and ( C ) mitotic index in melanoma tissue. Mean values ± SEM; statistical significance in comparison to melanoma tissue of control animals (* p value) and between the treated groups.

    Article Snippet: After deparaffinization and rehydration, epitope retrieval was performed by microwave treatment in citrate buffer for 5 min. After cooling and rinsing with distilled water and PBS, nonspecific binding was blocked by using 5% bovine serum albumin (BSA) at room temperature, for 30 min. A rabbit polyclonal antibody against PCNA was used (eBioscience, #60053, 1/100, overnight, at 4 °C), followed by thorough washing in PBS with Tween 20.

    Techniques: Inhibition, In Vivo, Staining, Comparison, Control